![]() Initial operations were performed in 20 mm tris at ph 7.5, 1 mm edta, and 0.1% sds using the bioforce mfp-3d plus afm. after that, we defined the region of interest to be the entire field of view that is the area covered by the microspheres (i.e. this step is used to correct the image for chromatic aberration, which is the widening of the focal spot due to the different wavelengths of light. we used the image processing software, nanoscope, to compute the optical resolution, which is the half of the diagonal of the measured profile of the microspheres and is defined as the full width at half-maximum (fwhm). to this end, we acquired a calibration image of fluorescent microspheres of known diameter (d = 3.5μm) at the center of the acoustofluidic chamber. first, we corrected the focal plane of the two cameras used in the acoustofluidic nanoscope. ![]() ![]() Nanoscope Software 6.13 Download Google #TOP# □įor the extraction of the optical resolution, we used the open-source image analysis software, nanoscope. ![]()
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